3KV1
Crystal Structure of Putative Sugar-Binding Domain of Transcriptional Repressor from Vibrio fischeri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.382, 43.474, 47.536 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 23.400 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 28012 | |
| <I/σ(I)> | 17 | 2.7 |
| Completeness [%] | 98.5 | 82.7 |
| Redundancy | 3.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 0.1 M CHES pH 9.5, 30% (v/v) PEG-400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
