3KUZ
Crystal structure of the ubiquitin like domain of PLXNC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.246, 72.246, 116.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.251 |
| Rwork | 0.250 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | superimposed ensemble of PDB entries 3h6n and 3ig3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.466 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.180 | 2.300 |
| Rmerge | 0.187 | 0.057 | 0.963 |
| Number of reflections | 14277 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 99.6 | 98.4 | 98.3 |
| Redundancy | 9 | 8.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 2.0M ammonium sulfate, 0.2M sodium chloride, 0.1M HEPES, 1:100 w/w chymotrypsin, pH 7.5, vapor diffusion, sitting drop, temperature 291K |
