3KUQ
Crystal structure of the DLC1 RhoGAP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-05 |
| Detector | ADSC Q315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.577, 97.502, 92.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.750 - 2.300 |
| R-factor | 0.21821 |
| Rwork | 0.216 |
| R-free | 0.26028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fk2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.437 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.685 | |
| Number of reflections | 12307 | |
| Completeness [%] | 98.2 | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | 30% PEG-3350, 0.2M sodium chloride, 5% glycerol, 0.02M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 291K, VAPOR DIFFUSION, HANGING DROP |
