3KUJ
Crystal structure of C-terminal domain of PABPC1 in complex with binding region of eRF3a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 45.217, 50.802, 32.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.770 - 1.400 |
| R-factor | 0.19713 |
| Rwork | 0.196 |
| R-free | 0.22331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | 0.375 |
| Number of reflections | 14231 | |
| <I/σ(I)> | 19.2 | 6.1 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 7.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 1.4M ammonium sulfate, 0.1M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
