3KU7
Crystal structure of Helicobacter pylori MinE, a cell division topological specificity factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 64 |
Unit cell lengths | 70.840, 70.840, 65.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.780 - 2.800 |
R-factor | 0.268 |
Rwork | 0.268 |
R-free | 0.29800 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 4582 | |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES-NaOH (pH 6.5), 26% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES-NaOH (pH 6.5), 26% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |