3KU6
Crystal structure of a H2N2 influenza virus hemagglutinin, 226L/228G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97650 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.484, 70.484, 236.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 1.750 |
| R-factor | 0.19253 |
| Rwork | 0.191 |
| R-free | 0.22355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.071 | 0.330 |
| Number of reflections | 66916 | |
| <I/σ(I)> | 16.3 | 3.5 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 30% PEG 3000, 0.1M Tris, pH 7.8, vapor diffusion, sitting drop, temperature 295K |
