3KU3
Crystal structure of a H2N2 influenza virus hemagglutinin, avian like
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.252, 70.252, 236.879 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.200 - 1.600 |
| R-factor | 0.20074 |
| Rwork | 0.199 |
| R-free | 0.23037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.406 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.000 | 32.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.062 | 0.046 | 0.676 |
| Number of reflections | 103348 | ||
| <I/σ(I)> | 17 | ||
| Completeness [%] | 98.0 | 99.7 | 82.4 |
| Redundancy | 11.7 | 16.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 28% PEG 3000, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
