3KTW
Crystal structure of the SRP19/S-domain SRP RNA complex of Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.307, 79.338, 114.067 |
Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
Resolution | 64.680 - 3.200 |
R-factor | 0.242 |
Rwork | 0.240 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lng |
RMSD bond length | 0.015 |
RMSD bond angle | 1.619 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.700 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 20578 | |
<I/σ(I)> | 13.6 | 3.3 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 100 mM Na cacodylate, 400 mM KCl, 10 mM CaCl2, 15% (w/v) PEG4000, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na cacodylate | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | PEG4000 | ||
5 | 1 | 2 | Na cacodylate | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | PEG4000 |