3KTS

CRYSTAL STRUCTURE OF GLYCEROL UPTAKE OPERON ANTITERMINATOR REGULATORY PROTEIN FROM LISTERIA MONOCYTOGENES STR. 4b F2365

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	93
Detector technology	CCD
Collection date	2009-11-10
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	137.603, 110.599, 130.971
Unit cell angles	90.00, 116.44, 90.00

Refinement procedure

Resolution	20.000 - 2.750
R-factor	0.21964
Rwork	0.218
R-free	0.27150
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.220
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.3.0034)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge		0.920
Number of reflections	45680
<I/σ(I)>	6.6	0.7
Completeness [%]	99.7	99.9
Redundancy	2.1	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	0.15M DL MALIC ACID PH 7, 20% PEG3350, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K