3KTO
Crystal structure of response regulator receiver protein from Pseudoalteromonas atlantica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.333, 116.333, 169.701 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.550 - 1.980 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.083 | 0.369 |
| Number of reflections | 30894 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 100.0 | 88.5 |
| Redundancy | 24 | 24.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M Sodium chloride, 0.1M HEPES pH 7.5, 25% w/v Polyethylene Glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
