3KTN
Crystal Structure of a putative 2-Keto-3-deoxygluconate Kinase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 145.564, 145.564, 126.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.590 - 2.260 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 41573 | |
| <I/σ(I)> | 13.5 | 11.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 42.9 | 41.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.2M Ammonium Citrate pH 7.0 8% W/V Trimethylamine N-oxide dihydrate 20% W/V PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
