3KTH
Structure of ClpP from Bacillus subtilis in orthorombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.293, 108.117, 152.644 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
Rwork | 0.248 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.186 |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 28722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 8 | 295 | 0.1M sodium citrate(pH 5.6), 0.1M lithium sulfate, 12% PEG 4000, hanging drop, temperature 295K |