3KTF
Structure of the N-terminal BRCT domain of human microcephalin (MCPH1).
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.069, 39.274, 106.639 |
Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.139 |
Rwork | 0.138 |
R-free | 0.19500 |
Structure solution method | SAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.046 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.620 |
High resolution limit [Å] | 1.600 | 1.550 |
Rmerge | 0.059 | 0.397 |
Number of reflections | 36909 | |
<I/σ(I)> | 46.07 | 2.95 |
Completeness [%] | 88.9 | 64.1 |
Redundancy | 6.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 295 | 0.2M sodium succinate, 15% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |