3KT9
Aprataxin FHA Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.949, 40.554, 42.477 |
| Unit cell angles | 90.00, 110.24, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.650 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.999 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 1.720 |
| High resolution limit [Å] | 1.650 | 3.290 | 1.650 |
| Rmerge | 0.052 | 0.034 | 0.299 |
| Number of reflections | 11553 | ||
| <I/σ(I)> | 19.3 | ||
| Completeness [%] | 90.1 | 98.1 | 63.6 |
| Redundancy | 5.1 | 5.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Ammonium fluoride, PEG 3350, pH 7.5, vapor diffusion, hanging drop, temperature 291K |
