3KT7

Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23990
Spacegroup name	P 3 2 1
Unit cell lengths	136.293, 136.293, 79.832
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.770
R-factor	0.18
Rwork	0.179
R-free	0.20500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.770	4.800	1.770
Rmerge	0.061	0.039	0.419
Number of reflections	82927
<I/σ(I)>	17.5
Completeness [%]	99.8	99.4	96.5
Redundancy	20	20.9	18.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	287	200mM lithium sulfate, 100mM Tris-HCl, 25%(w/v) PEG 3350, 0.14mM ferrous ascorbate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 287K