3KT5
Crystal Structure of N88S mutant HIV-1 Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976180 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.170, 62.170, 83.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.869 - 1.801 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.869 | 1.910 |
| High resolution limit [Å] | 1.801 | 1.800 |
| Rmerge | 0.067 | 0.537 |
| Number of reflections | 16807 | |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 5.4 | 5.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 1-5% saturated Ammonium Sulfate, 200/100mM Phosphate/Citrate Buffer, pH 6.2, vapor diffusion, hanging drop, temperature 298K |
