3KT4

Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97954
Spacegroup name	P 3 2 1
Unit cell lengths	136.293, 136.293, 83.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.730
R-factor	0.193
Rwork	0.190
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.245
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.830
High resolution limit [Å]	2.730	5.880	2.730
Rmerge	0.165	0.110	0.655
Number of reflections	23991
<I/σ(I)>	8.5
Completeness [%]	100.0	100	100
Redundancy	30.5	28.7	28.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	287	200mM lithium sulfate, 100mM Tris-HCl, 25%(w/v) PEG 3350, 0.14mM ferrous ascorbate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 287K