3KSN

Crystal structure of the lipoprotein localization factor, LolA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0809
Spacegroup name	P 32 2 1
Unit cell lengths	60.357, 60.357, 79.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.577 - 1.650
R-factor	0.1961
Rwork	0.194
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1au8
RMSD bond length	0.006
RMSD bond angle	1.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_159))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.047	0.534
Number of reflections	20611
<I/σ(I)>	39.5	4.47
Completeness [%]	99.6	99.7
Redundancy	9.8	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	277	25% PEG 1000, 20% PEG 8000, 0.1 M sodium acetate, dehydrated over 1M ammonium sulfate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K