3KSH

Structure of fRMsr of Staphylococcus aureus (oxidized form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-31
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2386
Spacegroup name	P 61 2 2
Unit cell lengths	89.993, 89.993, 88.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.110 - 1.500
R-factor	0.292
Rwork	0.245
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhm
RMSD bond angle	0.004
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.2)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	33964
Completeness [%]	87.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2M ammonium sulfate, 7% 2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K