3KSB
Detailed structural insight into the DNA cleavage complex of type IIA topoisomerases (re-sealed form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9763 |
Spacegroup name | P 32 |
Unit cell lengths | 116.757, 116.757, 182.811 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.318 - 3.500 |
R-factor | 0.1859 |
Rwork | 0.181 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k9f |
RMSD bond length | 0.027 |
RMSD bond angle | 1.441 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.065 | 0.400 |
Number of reflections | 34407 | |
<I/σ(I)> | 21.958 | 4.325 |
Completeness [%] | 97.7 | 100 |
Redundancy | 6.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 4-5% isopropanol, optimised mixture of salts, 50mM Na cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |