3KR9
Crystal structure of Streptococcus pneumoniae Sp1610, a putative tRNA (m1A22) methyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Wavelength(s) | 0.97888, 0.97917, 0.96395 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.807, 58.668, 142.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.430 - 2.000 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.695 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.430 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 20787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 298 | 2.0M ammonium sulfate, 0.1M tri-sodium citrate pH5.6, 0.2M sodium/potassium tartrate, hanging drop, temperature 298K |
