3KQR
The structure of serum amyloid p component bound to phosphoethanolamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.860, 69.860, 102.270 |
Unit cell angles | 90.00, 96.95, 90.00 |
Refinement procedure
Resolution | 36.811 - 1.500 |
R-factor | 0.142 |
Rwork | 0.141 |
R-free | 0.16100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.579 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.812 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.053 | 0.180 |
Number of reflections | 208934 | |
<I/σ(I)> | 16.8 | 4 |
Completeness [%] | 98.7 | 97.7 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.06M TRIS-HCL pH8, 16% PEG 550MME, 0.01M CaCl2, 0.14M NaCl, 0.1% NaN3,14.2mg/ml Protein, 0.05M Ligand, VAPOR DIFFUSION, HANGING DROP, temperature 298K |