3KQG

Trimeric Structure of Langerin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.92748
Spacegroup name	P 1 21 1
Unit cell lengths	80.780, 77.460, 85.920
Unit cell angles	90.00, 94.42, 90.00

Refinement procedure

Resolution	47.958 - 2.300
R-factor	0.1849
Rwork	0.182
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	a model from a low resolution data set.
RMSD bond length	0.005
RMSD bond angle	0.818
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	COMO
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.750	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.079	0.340
Number of reflections	46977
<I/σ(I)>	11.2	4.2
Completeness [%]	99.6	99.1
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	Protein solution: 10 mg/ml protein, 10 mM Tris pH 8.0, 25 mM NaCl. Reservoir solution: 15% PEG3350, 0.2M Ammonium chloride., VAPOR DIFFUSION, HANGING DROP, temperature 295K