3KPT
Crystal structure of BcpA, the major pilin subunit of Bacillus cereus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97929, 0.97946, 0.96863 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.552, 73.862, 201.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.607 - 2.102 |
R-factor | 0.2327 |
Rwork | 0.231 |
R-free | 0.27670 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.699 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 48052 | |
Completeness [%] | 88.2 | 57.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350, 0.2M Calcium acetate, 0.05M Tris-HCl pH 7.5, 0.15M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |