3KPA

Ubiquitin fold modifier conjugating enzyme from Leishmania major (probable)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9784
Spacegroup name	P 1 21 1
Unit cell lengths	57.274, 34.133, 126.774
Unit cell angles	90.00, 99.32, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.217
Rwork	0.214
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2in1
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	0.394
Number of reflections	23133
<I/σ(I)>	11.4	2.2
Completeness [%]	91.4	70.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	protein buffer 25 mM HEPES pH 7.5, 120 mM NaCl; crystallization buffer 100 mM Na Cacodylate, 300 mM Ammonium Sulfate,, 30% PEG 4000, vapor diffusion, temperature 293K