3KOZ
Crystal Structure of ornithine 4,5 aminomutase in complex with ornithine (Anaerobic)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.072 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.520, 233.220, 124.140 |
Unit cell angles | 90.00, 103.43, 90.00 |
Refinement procedure
Resolution | 60.373 - 2.800 |
R-factor | 0.1885 |
Rwork | 0.185 |
R-free | 0.25380 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.440 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.082 | 0.413 |
Number of reflections | 99008 | |
<I/σ(I)> | 9.3 | 2.4 |
Completeness [%] | 98.9 | 99.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | A protein solution (8 mg/ml 4,5-OAM, 5 mM 2-mercaptoethanol, 10 mM Tris HCl pH 8.0, 2 mM PLP, and 2 mM AdoCbl) was mixed in a 1:1 ratio with precipitant solution [(0.1 M Tris HCl, pH 8.0, 0.2 M MgCl2, 25% (wt/vol) polyethylene glycol 2000 mono-methylether] under red light at room temperature, VAPOR DIFFUSION, SITTING DROP, temperature 294K |