3KOW
Crystal Structure of ornithine 4,5 aminomutase backsoaked complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.180, 229.010, 123.920 |
| Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
| Resolution | 120.860 - 2.900 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.27900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.611 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.860 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.108 | 0.278 |
| Number of reflections | 72733 | |
| <I/σ(I)> | 8.3 | 3.4 |
| Completeness [%] | 97.9 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 294 | A protein solution (8 mg/ml 4,5-OAM, 5 mM 2-mercaptoethanol, 10 mM Tris HCl pH 8.0, 2 mM PLP, and 2 mM AdoCbl) was mixed in a 1:1 ratio with precipitant solution [(0.1 M Tris HCl, pH 8.0, 0.2 M MgCl2, 25 % (wt/vol) polyethylene glycol 2000 mono-methylether] under red light at room temperature., VAPOR DIFFUSION, SITTING DROP, temperature 294K |
