3KOS
Structure of the AmpR effector binding domain from Citrobacter freundii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-08-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979594, 0.979741, 1.019867 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.280, 34.190, 49.980 |
| Unit cell angles | 90.00, 102.77, 90.00 |
Refinement procedure
| Resolution | 28.071 - 1.830 |
| R-factor | 0.1735 |
| Rwork | 0.170 |
| R-free | 0.20220 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.4_129)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.920 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.082 | 0.446 |
| Number of reflections | 18564 | |
| <I/σ(I)> | 17.3 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 10% PEG 4000, 10% glycerol, 100mM MES pH5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
