3KOM

Crystal structure of apo transketolase from Francisella tularensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	110
Detector technology	CCD
Collection date	2009-10-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	86.490, 109.290, 128.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.040 - 1.600
R-factor	0.165
Rwork	0.163
R-free	0.19900
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.356
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CRANK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.081	0.626
Number of reflections	306041
<I/σ(I)>	11	2.6
Completeness [%]	99.0	97.6
Redundancy	7.1	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	30% PEG MME 2000, 200mM Ammonium acetate, 100mM Sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K