3KOL
Crystal structure of a glyoxalase/dioxygenase from Nostoc punctiforme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 50.382, 50.382, 138.026 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.095 | 0.357 |
Number of reflections | 14757 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 54.8 | 55.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Ammonium Sulfate; MES pH 6.5; Cobalt (II) Chloride hexahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 290K |