3KOJ
Crystal structure of the SSB domain of Q5N255_SYNP6 protein from Synechococcus sp. Northeast Structural Genomics Consortium Target SnR59a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.843, 113.028, 73.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.691 - 1.895 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.21830 |
| Structure solution method | SAD |
| RMSD bond length | 0.043 |
| RMSD bond angle | 1.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.895 | 1.900 |
| Rmerge | 0.105 | 0.666 |
| Number of reflections | 44952 | |
| <I/σ(I)> | 21.2 | 2.7 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbutch under parafin oil | 7.5 | 291 | 4M sodium chloride, 0.1M HEPES pH 7.5 , Microbutch under parafin oil, temperature 291K |
