3KOB
DTD from Plasmodium falciparum in complex with D-Glutamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.948, 54.656, 92.619 |
| Unit cell angles | 106.86, 102.10, 94.35 |
Refinement procedure
| Resolution | 30.000 - 2.990 |
| R-factor | 0.201 |
| Rwork | 0.196 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.518 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.090 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.055 | 0.408 |
| Number of reflections | 18534 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 96.0 | 79.4 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 0.1 M MES, pH 6.2-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
