3KO8

Crystal Structure of UDP-galactose 4-epimerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	100.803, 100.803, 76.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.250 - 1.800
R-factor	0.205
Rwork	0.204
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ehe
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	36921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	6.6	293	2-ethoxy ethanol, pH 6.6, sitting drop, temperature 293K