3KO1
Cystal structure of thermosome from Acidianus tengchongensis strain S5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.674, 283.042, 160.750 |
| Unit cell angles | 90.00, 133.90, 90.00 |
Refinement procedure
| Resolution | 48.390 - 3.700 |
| R-factor | 0.277 |
| Rwork | 0.277 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q3r |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.117 | 0.476 |
| Number of reflections | 76576 | |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-Hcl, 1.15M Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
