3KNW
Crystal structure of a putative transcriptional regulator (TetR/AcrR family member) from putative transcriptional regulator (TetR/AcrR family)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.741, 73.768, 106.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.450 |
| R-factor | 0.21265 |
| Rwork | 0.210 |
| R-free | 0.26327 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.685 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.088 | 0.520 |
| Number of reflections | 18816 | |
| <I/σ(I)> | 23.5 | 2.8 |
| Completeness [%] | 98.9 | 96.4 |
| Redundancy | 5.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M TRIS, 20% PEG-1000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
