3KN7
Crystal Structure of Haemophilus influenzae Y195A mutant Holo Ferric ion-Binding Protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-06 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.464, 75.822, 34.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.000 - 1.710 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 30268 | |
| <I/σ(I)> | 15.16 | 6.67 |
| Completeness [%] | 96.8 | 96.75 |
| Redundancy | 6.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 34% PEG 550 MME, 0.1 M Tris pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
