3KN3
Crystal Structure of LysR Substrate Binding Domain (25-263) of Putative Periplasmic Protein from Wolinella succinogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.784, 81.290, 87.910 |
| Unit cell angles | 90.00, 103.32, 90.00 |
Refinement procedure
| Resolution | 34.527 - 2.412 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.530 | 2.450 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 29443 | |
| <I/σ(I)> | 11.5 | 3.9 |
| Completeness [%] | 99.3 | 91.7 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 289 | 0.1 M Citric acid pH 3.5, 25 % w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
