3KMU
Crystal structure of the ILK/alpha-parvin core complex (apo)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97880, 0.97900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.380, 117.715, 47.344 |
Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
Resolution | 58.820 - 1.800 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.484 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.820 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 94370 | |
<I/σ(I)> | 28.2 | 2.3 |
Completeness [%] | 99.9 | 96.9 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 277 | 0.05 M Bis-Tris, 12% PEG 5000 MME, 5% 1-Propyl alcohol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |