3KMA

Crystal Structure of vSET under Condition A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	160
Detector technology	CCD
Collection date	2009-08-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	60.403, 60.403, 102.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.25
Rwork	0.234
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.600
Number of reflections	27966
Completeness [%]	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	20% PEG 4000, 0.1 M Bis-Tris pH 5, 0.4 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K