3KM2
As-isolated TOMATO CHLOROPLAST SUPEROXIDE DISMUTASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2823 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 142.033, 144.523, 192.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.978 - 3.100 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hl5 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.318 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 73425 | |
<I/σ(I)> | 13.5 | 3.2 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 65% MPD, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |