3KLZ

Pentameric formate channel with formate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-24
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	99.779, 100.456, 192.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.500
R-factor	0.175
Rwork	0.173
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.026
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	62189
<I/σ(I)>	14.6
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.02M MOPS, PH 6.8, 29% (W/V) PEG 550MME, 120MM SODIUM FORMATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K