3KLX
Crystal structure of native abscisic acid receptor PYL3
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2009-10-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.964, 86.964, 154.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.25298 |
Rwork | 0.252 |
R-free | 0.26959 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.147 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 19933 | |
Completeness [%] | 95.8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 298 | 20mM tris, 2.0M ammonium sulfate, pH 8, EVAPORATION, temperature 298K |
1 | EVAPORATION | 8 | 298 | 20mM tris, 2.0M ammonium sulfate, pH 8, EVAPORATION, temperature 298K |