3KLX

Crystal structure of native abscisic acid receptor PYL3

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	130
Detector technology	CCD
Collection date	2009-10-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	86.964, 86.964, 154.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.25298
Rwork	0.252
R-free	0.26959
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	19933
Completeness [%]	95.8	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	298	20mM tris, 2.0M ammonium sulfate, pH 8, EVAPORATION, temperature 298K
1	EVAPORATION	8	298	20mM tris, 2.0M ammonium sulfate, pH 8, EVAPORATION, temperature 298K