3KLT
Crystal structure of a vimentin fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0720, 1.8449, 1.8457 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.200, 30.100, 82.980 |
| Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
| Resolution | 40.473 - 2.700 |
| R-factor | 0.2935 |
| Rwork | 0.291 |
| R-free | 0.33110 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.440 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.039 |
| Number of reflections | 15461 |
| <I/σ(I)> | 15.2 |
| Completeness [%] | 95.5 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 27% PEG monoethylether 550, 27mM CaCl2, 7.5% (v/v) glycerol, 10mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
