3KLS
Structure of complement C5 in complex with SSL7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0379 |
Spacegroup name | P 31 |
Unit cell lengths | 143.875, 143.875, 241.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.047 - 3.600 |
R-factor | 0.2009 |
Rwork | 0.198 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3CU7 chain A residues 20-1510 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.658 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.047 | 3.800 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 64555 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 277 | Reservoir contains 50mM MgAc2, 50 mM MES pH 6.2, mixed 1:1 with protein, VAPOR DIFFUSION, temperature 277K |