3KLR
Bovine H-protein at 0.88 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.584, 41.295, 43.124 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 45.000 - 0.880 |
| Rwork | 0.112 |
| R-free | 0.13110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hpc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 0.890 |
| High resolution limit [Å] | 0.880 | 0.880 |
| Rmerge | 0.055 | 0.426 |
| Number of reflections | 115668 | |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 98.9 | 95.4 |
| Redundancy | 9.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 288 | 2.0M ammonium sulfate, 0.1M sodium citrate, pH 3.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
