3KLQ

Crystal Structure of the Minor Pilin FctB from Streptococcus pyogenes 90/306S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.980
Spacegroup name	P 65
Unit cell lengths	95.192, 95.192, 100.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.554 - 1.900
R-factor	0.1597
Rwork	0.159
R-free	0.18200
Structure solution method	SIR
RMSD bond length	0.006
RMSD bond angle	0.774
Data scaling software	SCALA
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.555	19.560	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.066	0.039	0.531
Total number of observations		9566	44910
Number of reflections	40679
<I/σ(I)>	19.2	13.6	1.4
Completeness [%]	99.9	97.1	100
Redundancy	7.6	7.5	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1M Na-citrate, 0.1M Na-cadoylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K