3KL4

Recognition of a signal peptide by the signal recognition particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-16
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	P 41 21 2
Unit cell lengths	91.883, 91.883, 133.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.420 - 3.500
R-factor	0.301
Rwork	0.301
R-free	0.32200
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.619
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.420	3.690
High resolution limit [Å]	3.500	3.500
Rmerge	0.081	0.762
Number of reflections	7683
<I/σ(I)>	29.2	5.1
Completeness [%]	99.9	100
Redundancy	26.7	27.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	295	5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K
1	VAPOR DIFFUSION	5.5	295	5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K
1	VAPOR DIFFUSION	5.5	295	5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K