3KKO
Crystal structure of M-Ras P40D/D41E/L51R in complex with GppNHp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-10 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.196, 192.248, 43.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.570 - 1.900 |
R-factor | 0.18584 |
Rwork | 0.184 |
R-free | 0.22192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x1s |
RMSD bond length | 0.010 |
RMSD bond angle | 1.198 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.099 | 0.282 |
Number of reflections | 52129 | |
<I/σ(I)> | 3.8 | 2.5 |
Completeness [%] | 99.2 | 94.8 |
Redundancy | 6.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Ammonium sulfate, sodium acetate, PEG-4000, glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |