3KKE
Crystal structure of a LacI family transcriptional regulator from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.599, 65.468, 116.034 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.477 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.367 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.371 |
| Number of reflections | 49080 | |
| <I/σ(I)> | 14.2 | 5 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 100mM sodium acetate pH 4.6, 30% PEG 4000, 200mM ammonium acetate, vapor diffusion, temperature 294K |
