3KKC
The crystal structure OF TetR transcriptional regulator from Streptococcus agalactiae 2603V
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940, 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.351, 128.681, 74.566 |
| Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
| Resolution | 48.148 - 2.500 |
| R-factor | 0.2119 |
| Rwork | 0.208 |
| R-free | 0.27880 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | |
| Number of reflections | 27427 | |
| <I/σ(I)> | 24.9 | 1.44 |
| Completeness [%] | 97.5 | 83.9 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Tri-sodium citrate dihydrate, 20% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
